目的 研究三叶青三氯甲烷部位的化学成分及其体外抗肿瘤活性。方法 利用硅胶,制备薄层板,LH-20等各种色谱技术分离单体化合物,通过NMR和质谱对化合物进行结构鉴定,并采用气-质联用色谱对三叶青三氯甲烷部位的其他成分进行分析,采用MTT法对分离得到的单体化合物进行抗肿瘤活性评价。结果 通过经典色谱法分离得到6个化合物,分析鉴定为β-谷甾醇(1),4-羟基-3-甲氧基苯甲醛(2),齐墩果酸(3),5-羟甲基糠醛(4),壬二酸(5)和香草酸(6),并通过GC-MS指认了22个化合物。结论 化合物2~6首次从三叶青中分离得到。化合物1和3对Hela229有较强的细胞毒活性,IC50分别为40.78, 25.69 μg·mL-1。化合物3对A375有较强的细胞毒活性,IC50为69.87 μg·mL-1。三叶青三氯甲烷部位的体外抗肿瘤活性由多种成分共同作用产生。
Abstract
OBJECTIVE To investigate the chemical constituents in chloroform extraction of Tetrastigmatis hemsleyani diels et. Gilg and their antitumor activities. METHODS Various chromatography techniques such as column chromatography on silica gel, Sephadex LH-20 and preparative TLC were used to isolate and purify the compounds. Their structures were identified by1H-NMR,13C-NMR and MS. Their antitumor activities was tested by MTT method. Moreover, the other compounds of chloroform extraction were detected by GC-MS. RESULTS Six compounds were isolated by classic chromatography and identified as β-sitosterol(1), 4-hydroxy-3-methoxybenzaldehyde(2), oleanolic acid (3), 5-hydroxymethyl furfural(4), azelaic acid(5), vanillic acid(6). Twenty-two compounds were identified by GC-MS. CONCLUSION Compounds 2-6 are isolated from this plant for the first time. Compounds 1 and 3 shows strong cytotoxic activities against Hela229 with IC50 values of 40.78, 25.69 μg·mL-1, respectively. Compound 3 also showed strong cytotoxic activities against with IC50 values of 69.87 μg·mL-1. The result proved that antitumor activity of chloroform extraction of Tetrastigmatis hemsleyani diels et. Gilg is due to the contribution of multi-components.
关键词
三叶青 /
气质联用 /
抗肿瘤 /
4-羟基-3-甲氧基苯甲醛 /
5-羟甲基糠醛 /
香草酸
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Key words
Tetrastigmatis hemsleyani /
GC-MS /
antitumor /
4-hydroxy-3-methoxybenzaldehyde /
5-hydroxymethyl furfural /
vanillic acid
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中图分类号：
R284
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参考文献
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脚注
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基金
宁波市自然科学基金资助项目(2012A610181)
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